Microbial mechanisms of tolerance to weak acid stress

Microbial mechanisms of tolerance to weak acid stress

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Carboxylic acids are ubiquitous molecules found in microbial metabolic pathways and that have been explored for a wide array of applications including food preservation (e.g., acetic, propionic, benzoic, and sorbic acids), chemotherapy (e.g., the analgesic acetylsalicylic acid, the immunosuppressor mycophenolic acid or the antimalarial drugs artesunic and artemisinic acids) or agriculture (e.g., the herbicides 2, 4-dichlorophenoxyacetic acid). This Research Topic contributes to the understanding of the molecular mechanisms underlying adaptation to weak acid stress in microbes, a knowledge base that impacts the fields of Medicine, Health, Food Safety and the Environment.Synthesis of cDNA for real-time RT-PCR experiments, from total RNA samples, was performed using the MultiscribeTM reverse transcriptase kit (Applied Biosystems) and the 7500 RT-PCR Thermal Cycler Block (Applied Biosystems), following the manufacturera#39;s instructions. ... The RT-PCR reaction was carried out using a thermal cycler block (7500 Real-Time PCR System a€“ Applied Biosystems ). Defaultanbsp;...

Title:Microbial mechanisms of tolerance to weak acid stress
Author: Nuno Pereira Mira, Miguel Cacho Teixeira
Publisher:Frontiers E-books - 2014-11-03

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